Samples were in that case homogenized and cytoplasmic and mitochondrial protein lysates were separated by differential centrifugation (entire cell lysates was centrifuged in 1000for 10 min to get the supernatant. discovered that EPI induced autophagy and apoptosis in both cell lines. The lysosomal inhibitor bafilomycin A1 inhibited mobile autophagy and improved EPI\activated apoptosis, because of … Continue reading Samples were in that case homogenized and cytoplasmic and mitochondrial protein lysates were separated by differential centrifugation (entire cell lysates was centrifuged in 1000for 10 min to get the supernatant